Sequences of primers, probes, shRNA, and siRNA oligonucleotides used
| PCR | |
| PRELI-Fwd1 | 5′-CGGAATTCCATGGTGAAGTATTTCCTGGGCCA-3′ |
| PRELI-Rev1 | 5′-TGCTCTAGACTACACAAACTGTTGCTGCTGCTG-3′ |
| PRELI-Rev2 | 5′-CGCGGATCCCTACACAAACTGTTGCTGCTGCTG-3′ |
| Taqman RT-PCR | |
| EF1α-Probe | 5′-(FAM)-AGCGCCGGCTATGCCCCTG-(TAMRA)-3′ |
| EF1α-Fwd | 5′-CTGAACCATCCAGGCCAAAT-3′ |
| EF1α-Rev | 5′-GCCGTGTGGCAATCCAAT-3′ |
| PRELI-Probe | 5′-(FAM)-CCGGGAAGCCTGGGTCTCCTCTAGCT-(TAMRA)-3′ |
| PRELI-Fwd | 5′-TGGCTGGACTGAAATCC-3′ |
| PRELI-Rev | 5′-CAGCTCTGGAGACACCAAAT-3′ |
| STAT6-Probe | 5′-(FAM)-CAGGACACCATCAAACCACTGCCAAA-(TAMRA)-3′ |
| STAT6-Fwd | 5′-TGGGCCGTGGCTTCAC-3′ |
| STAT6-Rev | 5′-CCGGAGACAGCGTTTGGT-3′ |
| siRNA/shRNA sequence | |
| Scramble | 5′-GCGCGCUUUGUAGGAUUCGUU-3′ |
| PRELI-siRNA1 | 5′-CCAAGACUAUGAAGGGUUUU-3′ |
| PRELI-siRNA2 | 5′-CCAAGGACCUCGCCAGCAAUU-3′ |
| PRELI-shRNA | 5′-GGAGAAGGCAAAGGAGACGUU-3′ |
| PCR | |
| PRELI-Fwd1 | 5′-CGGAATTCCATGGTGAAGTATTTCCTGGGCCA-3′ |
| PRELI-Rev1 | 5′-TGCTCTAGACTACACAAACTGTTGCTGCTGCTG-3′ |
| PRELI-Rev2 | 5′-CGCGGATCCCTACACAAACTGTTGCTGCTGCTG-3′ |
| Taqman RT-PCR | |
| EF1α-Probe | 5′-(FAM)-AGCGCCGGCTATGCCCCTG-(TAMRA)-3′ |
| EF1α-Fwd | 5′-CTGAACCATCCAGGCCAAAT-3′ |
| EF1α-Rev | 5′-GCCGTGTGGCAATCCAAT-3′ |
| PRELI-Probe | 5′-(FAM)-CCGGGAAGCCTGGGTCTCCTCTAGCT-(TAMRA)-3′ |
| PRELI-Fwd | 5′-TGGCTGGACTGAAATCC-3′ |
| PRELI-Rev | 5′-CAGCTCTGGAGACACCAAAT-3′ |
| STAT6-Probe | 5′-(FAM)-CAGGACACCATCAAACCACTGCCAAA-(TAMRA)-3′ |
| STAT6-Fwd | 5′-TGGGCCGTGGCTTCAC-3′ |
| STAT6-Rev | 5′-CCGGAGACAGCGTTTGGT-3′ |
| siRNA/shRNA sequence | |
| Scramble | 5′-GCGCGCUUUGUAGGAUUCGUU-3′ |
| PRELI-siRNA1 | 5′-CCAAGACUAUGAAGGGUUUU-3′ |
| PRELI-siRNA2 | 5′-CCAAGGACCUCGCCAGCAAUU-3′ |
| PRELI-shRNA | 5′-GGAGAAGGCAAAGGAGACGUU-3′ |
Fwd indicates forward; Rev, reverse; FAM, 6-carboxyfluorescein; and TAMRA, 6-carboxytetramethylrhodamine.