Spectratype analysis of the responding T-cell repertoires in the MLC and transplant recipients
| Vβ . | Patient no. . | |||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 . | 2 . | 3 . | 4 . | 5 . | 6 . | 7 . | 8 . | 9 . | 10 . | 11 . | 12 . | 13 . | 14 . | 15 . | 16 . | 17 . | 18 . | |
| 1 | − | + | NO | NO | + | + | + | NO | + | NO | + | ND | − | + | ND | ND | NO | + |
| 2 | ND | + | + | + | + | + | + | − | + | NO | − | + | + | NP | NO | + | + | + |
| 3 | ND | ND | + | ND | NO | + | − | − | + | + | + | + | − | + | + | NO | + | + |
| 4 | + | + | + | + | NP | + | ND | + | + | + | NO | NP | + | NO | + | ND | NO | NP |
| 5 | − | + | − | + | − | ND | NO | + | + | ND | + | + | + | NP | NP | ND | − | + |
| 6 | + | ND | NP | + | + | + | NP | NO | − | + | + | NO | + | + | ND | ND | − | + |
| 7 | ND | + | − | NO | + | + | + | ND | − | ND | + | NO | + | + | ND | + | − | ND |
| 8 | NO | NO | + | + | + | − | ND | − | + | ND | + | + | − | ND | + | + | + | + |
| 9 | + | + | + | + | + | + | + | − | + | + | + | + | − | + | + | NP | − | + |
| 11 | ND | + | NP | + | − | + | + | ND | − | + | + | + | + | + | + | ND | + | ND |
| 12 | + | ND | + | ND | + | NP | ND | − | ND | + | ND | NP | + | + | ND | ND | ND | NO |
| 13 | + | − | − | + | + | ND | + | + | + | + | + | + | − | + | + | + | ND | + |
| 14 | + | + | ND | ND | + | − | + | + | + | ND | ND | ND | + | + | ND | NO | + | + |
| 15 | NO | + | + | + | + | + | + | NO | + | + | − | + | + | + | − | ND | + | NO |
| 16 | NP | + | NP | − | + | + | NO | ND | + | + | ND | + | NO | NO | NP | + | + | + |
| 17 | + | + | + | NO | + | + | NP | + | − | ND | + | + | + | + | ND | + | + | NP |
| 18 | + | + | ND | NO | + | + | ND | ND | + | ND | NP | ND | ND | NO | ND | ND | NO | ND |
| 19 | + | NP | + | ND | + | + | + | − | + | ND | + | + | + | + | + | ND | + | NO |
| 21 | + | + | ND | + | + | + | ND | ND | + | ND | + | ND | ND | ND | ND | + | + | ND |
| 22 | ND | ND | ND | ND | + | NP | − | ND | + | ND | ND | ND | ND | + | ND | ND | + | NP |
| 23 | + | − | + | + | NP | − | + | + | NO | − | NO | + | + | NP | − | + | + | + |
| % + | 68.8 | 76.5 | 58.8 | 68.8 | 76.2 | 73.7 | 62.5 | 40.0 | 75.0 | 75.0 | 70.6 | 75.0 | 66.7 | 68.4 | 58.3 | 72.7 | 63.2 | 64.7 |
| % − | 12.5 | 11.8 | 17.6 | 6.3 | 9.5 | 15.8 | 12.5 | 40.0 | 20.0 | 8.3 | 11.8 | 0.0 | 27.8 | 0.0 | 16.7 | 0.0 | 21.1 | 0.0 |
| % NO | 18.8 | 5.9 | 5.9 | 25.0 | 4.8 | 0.0 | 12.5 | 20.0 | 5.0 | 16.7 | 11.8 | 12.5 | 5.6 | 15.8 | 8.3 | 18.2 | 15.8 | 17.6 |
| % NP | 6.3 | 5.9 | 17.6 | 0.0 | 9.5 | 10.5 | 12.5 | 0.0 | 0.0 | 0.0 | 5.9 | 12.5 | 0.0 | 15.8 | 16.7 | 9.1 | 0.0 | 17.6 |
| Vβ . | Patient no. . | |||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 . | 2 . | 3 . | 4 . | 5 . | 6 . | 7 . | 8 . | 9 . | 10 . | 11 . | 12 . | 13 . | 14 . | 15 . | 16 . | 17 . | 18 . | |
| 1 | − | + | NO | NO | + | + | + | NO | + | NO | + | ND | − | + | ND | ND | NO | + |
| 2 | ND | + | + | + | + | + | + | − | + | NO | − | + | + | NP | NO | + | + | + |
| 3 | ND | ND | + | ND | NO | + | − | − | + | + | + | + | − | + | + | NO | + | + |
| 4 | + | + | + | + | NP | + | ND | + | + | + | NO | NP | + | NO | + | ND | NO | NP |
| 5 | − | + | − | + | − | ND | NO | + | + | ND | + | + | + | NP | NP | ND | − | + |
| 6 | + | ND | NP | + | + | + | NP | NO | − | + | + | NO | + | + | ND | ND | − | + |
| 7 | ND | + | − | NO | + | + | + | ND | − | ND | + | NO | + | + | ND | + | − | ND |
| 8 | NO | NO | + | + | + | − | ND | − | + | ND | + | + | − | ND | + | + | + | + |
| 9 | + | + | + | + | + | + | + | − | + | + | + | + | − | + | + | NP | − | + |
| 11 | ND | + | NP | + | − | + | + | ND | − | + | + | + | + | + | + | ND | + | ND |
| 12 | + | ND | + | ND | + | NP | ND | − | ND | + | ND | NP | + | + | ND | ND | ND | NO |
| 13 | + | − | − | + | + | ND | + | + | + | + | + | + | − | + | + | + | ND | + |
| 14 | + | + | ND | ND | + | − | + | + | + | ND | ND | ND | + | + | ND | NO | + | + |
| 15 | NO | + | + | + | + | + | + | NO | + | + | − | + | + | + | − | ND | + | NO |
| 16 | NP | + | NP | − | + | + | NO | ND | + | + | ND | + | NO | NO | NP | + | + | + |
| 17 | + | + | + | NO | + | + | NP | + | − | ND | + | + | + | + | ND | + | + | NP |
| 18 | + | + | ND | NO | + | + | ND | ND | + | ND | NP | ND | ND | NO | ND | ND | NO | ND |
| 19 | + | NP | + | ND | + | + | + | − | + | ND | + | + | + | + | + | ND | + | NO |
| 21 | + | + | ND | + | + | + | ND | ND | + | ND | + | ND | ND | ND | ND | + | + | ND |
| 22 | ND | ND | ND | ND | + | NP | − | ND | + | ND | ND | ND | ND | + | ND | ND | + | NP |
| 23 | + | − | + | + | NP | − | + | + | NO | − | NO | + | + | NP | − | + | + | + |
| % + | 68.8 | 76.5 | 58.8 | 68.8 | 76.2 | 73.7 | 62.5 | 40.0 | 75.0 | 75.0 | 70.6 | 75.0 | 66.7 | 68.4 | 58.3 | 72.7 | 63.2 | 64.7 |
| % − | 12.5 | 11.8 | 17.6 | 6.3 | 9.5 | 15.8 | 12.5 | 40.0 | 20.0 | 8.3 | 11.8 | 0.0 | 27.8 | 0.0 | 16.7 | 0.0 | 21.1 | 0.0 |
| % NO | 18.8 | 5.9 | 5.9 | 25.0 | 4.8 | 0.0 | 12.5 | 20.0 | 5.0 | 16.7 | 11.8 | 12.5 | 5.6 | 15.8 | 8.3 | 18.2 | 15.8 | 17.6 |
| % NP | 6.3 | 5.9 | 17.6 | 0.0 | 9.5 | 10.5 | 12.5 | 0.0 | 0.0 | 0.0 | 5.9 | 12.5 | 0.0 | 15.8 | 16.7 | 9.1 | 0.0 | 17.6 |
+ indicates MLC and post both skewed in same peak, or neither skewed; −, MLC not skewed and post sample skewed; NO, no overlapping skewing (MLC and post sample skewed in different peak); NP, not predictive (MLC skewed and post sample not skewed); and ND, not determined.