Table 1 Primers used for RT PCR Gene... | American Society of Hematology

Table 1

Primers used for RT PCR

Gene name	Forward primer 5′→3′	Reverse primer 5′→3′	Product length, bp	Annealing temperature, °C
BMP-4	gactacatgcgggatctttacc	cccatagtttggctgcttctc	459	55
BMPR1b	gacctacaccctacactgcc	actttcccatccaaacttcc	355	51.5
BMPR1a	gcaattgctcatcgagacc	cgaagctgtagatgtcagcc	232	55
BMPR2	aatgcagccataagcgagg	tggtactctggtacggattcc	188	55
Brachyury	gcgggaaagagcctgcagta	ttccccgttcacgtacttcc	300	55
Wnt3	ggccatgaacaagcacaacaa	tgccgtgggaggtgacatt	368	58
Mixl1	ggtaccccgacatccactt	tggaaggatttcccactctg	120	55
αMHC	gtcattgctgaaaccgagaatg	gcaaagtactggatgacacgct	423	57
cTnT	ggcagcggaagaggatgctgaa	gaggcaccaagttgggcatgaacga	152	57
GATA4	gacgggtcactatctgtgcaac	agacatcgcactgactgagaac	475	60
MEF2	agattacgaggattatggatgaacgt	acctgcacttggaggtcgatgtg	563	56
Nkx2.5	cttcaagccagaggcctacg	ccgcctctgtcttcttcagc	233	55
SCL	tctctcggcagcgggttcttt	ccaggcggaggatctcattctt	259	58
CD34	cctaagtgacatcaaggcagaa	gcaaggagcagggagcata	201	55
γ-Globin	cgcttctggaacgtctgaggttat	ccaggagcttgaagttctcaggat	370	59
ϵ-Globin	cactagcctgtggagcaagatgaa	aatcaccatcacgttacccaggag	304	59
GAPDH	aatcccatcaccatcttcc	catcacgccacagtttcc	382	55

Gene name	Forward primer 5′→3′	Reverse primer 5′→3′	Product length, bp	Annealing temperature, °C
BMP-4	gactacatgcgggatctttacc	cccatagtttggctgcttctc	459	55
BMPR1b	gacctacaccctacactgcc	actttcccatccaaacttcc	355	51.5
BMPR1a	gcaattgctcatcgagacc	cgaagctgtagatgtcagcc	232	55
BMPR2	aatgcagccataagcgagg	tggtactctggtacggattcc	188	55
Brachyury	gcgggaaagagcctgcagta	ttccccgttcacgtacttcc	300	55
Wnt3	ggccatgaacaagcacaacaa	tgccgtgggaggtgacatt	368	58
Mixl1	ggtaccccgacatccactt	tggaaggatttcccactctg	120	55
αMHC	gtcattgctgaaaccgagaatg	gcaaagtactggatgacacgct	423	57
cTnT	ggcagcggaagaggatgctgaa	gaggcaccaagttgggcatgaacga	152	57
GATA4	gacgggtcactatctgtgcaac	agacatcgcactgactgagaac	475	60
MEF2	agattacgaggattatggatgaacgt	acctgcacttggaggtcgatgtg	563	56
Nkx2.5	cttcaagccagaggcctacg	ccgcctctgtcttcttcagc	233	55
SCL	tctctcggcagcgggttcttt	ccaggcggaggatctcattctt	259	58
CD34	cctaagtgacatcaaggcagaa	gcaaggagcagggagcata	201	55
γ-Globin	cgcttctggaacgtctgaggttat	ccaggagcttgaagttctcaggat	370	59
ϵ-Globin	cactagcctgtggagcaagatgaa	aatcaccatcacgttacccaggag	304	59
GAPDH	aatcccatcaccatcttcc	catcacgccacagtttcc	382	55