Table 1.

Diagnostic criteria for chronic neutrophilic leukemia32,33 

FeatureDiagnostic requirements
Peripheral blood counts Total WBC count ≥13/≥ 25 × 109/La
Segmented and band neutrophils ≥80% of WBCs
Myelocytes, metamyelocytes, and promyelocytes <10% of WBCs
Monocytes <10% of WBCs
Circulating blasts are rare or absent 
Morphology Absence of significant dysgranulopoiesis
Hypercellular bone marrow with increased myeloid:erythroid ratio and intact granulocytic maturation 
Genetics Activating CSF3R mutationb 
Exclusions No BCR::ABL1 rearrangement or rearrangement associated with myeloid/lymphoid neoplasms with eosinophilia and TK gene fusions
Not meeting criteria for PV, ET, or PMF 
FeatureDiagnostic requirements
Peripheral blood counts Total WBC count ≥13/≥ 25 × 109/La
Segmented and band neutrophils ≥80% of WBCs
Myelocytes, metamyelocytes, and promyelocytes <10% of WBCs
Monocytes <10% of WBCs
Circulating blasts are rare or absent 
Morphology Absence of significant dysgranulopoiesis
Hypercellular bone marrow with increased myeloid:erythroid ratio and intact granulocytic maturation 
Genetics Activating CSF3R mutationb 
Exclusions No BCR::ABL1 rearrangement or rearrangement associated with myeloid/lymphoid neoplasms with eosinophilia and TK gene fusions
Not meeting criteria for PV, ET, or PMF 
a

WHO fifth edition classification requires a minimal WBC of 25 × 109/L in all cases, while ICC allows a WBC ≥13 × 109/L in the presence of an activating CSF3R mutation.

b

In the absence of an activating CSF3R mutation, the neutrophilia must be persistent (≥3 months) and without an identifiable cause, and splenomegaly must be present.

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