Table 2.

How I use peg-IFN-α in practice

SafetyEfficacy
Before treatment • Exclusion of contraindications (per drug label)
• Search for potential cardiac or ophthalmologic abnormalities
• Screen for autoimmunity (at least antinuclear Ab) and TFT 
• Measure spleen size by echography
• Assess driver mutation allele burden
• If possible screen for additional mutations by NGS 
Treatment initiation • Start at low dose • Titration until CHR 
peg-IFN-α2a 45  µ/wk By 45  µ/2wk
Max dose 180  µ/wk 
ropegIFN-α2b 100  µ/2wk By 50  µ/2wk
Max dose 500  µ/2wk 
Monitoring during treatment • Screen for mood disturbances, thyroid dysfunction, type 1 diabetes
• CBC/mo
• LFT, TFT/6 mo
 
• CBC/mo
• Spleen size and mutation/y 
Dose adjustments According to potential toxicities Progressive decrease after 1 year in case of lasting CHR 
pegIFN-α2a  By 45  µ per injection every 6 months down to 45  µ/wk, then by expanding interval to every 2wk 
ropegIFN-α2b  By 50  µ per injection every 6 months down to 100  µ/2wk, then by expanding interval to every 4wk 
Discontinuation In case of intolerance In patients with at least 2 years of continuous CHR on IFN and JAK2 V617F VAF <10% 
SafetyEfficacy
Before treatment • Exclusion of contraindications (per drug label)
• Search for potential cardiac or ophthalmologic abnormalities
• Screen for autoimmunity (at least antinuclear Ab) and TFT 
• Measure spleen size by echography
• Assess driver mutation allele burden
• If possible screen for additional mutations by NGS 
Treatment initiation • Start at low dose • Titration until CHR 
peg-IFN-α2a 45  µ/wk By 45  µ/2wk
Max dose 180  µ/wk 
ropegIFN-α2b 100  µ/2wk By 50  µ/2wk
Max dose 500  µ/2wk 
Monitoring during treatment • Screen for mood disturbances, thyroid dysfunction, type 1 diabetes
• CBC/mo
• LFT, TFT/6 mo
 
• CBC/mo
• Spleen size and mutation/y 
Dose adjustments According to potential toxicities Progressive decrease after 1 year in case of lasting CHR 
pegIFN-α2a  By 45  µ per injection every 6 months down to 45  µ/wk, then by expanding interval to every 2wk 
ropegIFN-α2b  By 50  µ per injection every 6 months down to 100  µ/2wk, then by expanding interval to every 4wk 
Discontinuation In case of intolerance In patients with at least 2 years of continuous CHR on IFN and JAK2 V617F VAF <10% 

Ab, antibodies; CBC, complete blood count; LFT, liver function tests; NGS, next-generation sequencing; TFT, thyroid function tests.

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